Development of Detection Techniques for Amylose Content in Rice

Amylose content is an important index to evaluate rice cooking and eating quality. Therefore, the detection of amylose content in rice is the basic work for rice quality breeding. The most commonly used methods for the determination of rice amylose content include iodine colorimetry, amperometric titration, voltage titration, and gel filtration. The standard iodine blue colorimetric method (also known as “conventional method”) ")) The sample to be tested needs to be subjected to a series of destructive pretreatments such as rough, fine whitening, and comminution. After processing, the rice sample cannot be continuously planted, and the operation procedure is cumbersome and the test period is long, and it is impossible to satisfy the breeding worker on the early generations. As a result of the screening requirements, Gold Infrared Spectroscopy technology was later developed. It is a physical testing technology that has the features of no need for pretreatment of samples and fast and nondestructive detection. In the 1990s, foreign scholars used near-infrared spectroscopy to study the amylose content, water content, and protein content of rice grain, brown rice, and polished rice. Near-infrared quantitative analysis was included in the World Association of Grain Chemistry and Technology Standards. The standards of the American Cereal Chemistry Association have become one of the testing methods for the world's food analysis standards, but there are shortcomings such as technical difficulties, slow development, and the need to establish relevant model libraries when measuring.

Today, amylose measuring instrument is used to determine the content of amylose in crops. The amylose analyzer using laser light source and the image analysis and recognition software system of CCD rice appearance quality are innovative achievements and fill the instrumentation in our country. The blank in the field of rice quality testing is the first in the country and it is an international advanced level. For example, our company's DPCZ-II Amylose Detector has been tested by the Ministry of Agriculture's Grain Quality Supervision, Inspection and Test Center and is widely used in rice, corn, wheat and other crops.

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